Jul 10

Supercharging reagents – revving up peptide LC-MS analyses

Over the past 5 years, LC-MS has (arguably) become the technique of choice to quantify peptides in biological matrices. The benefits of using LC-MS as a quantitative technique are significant, however, there is one drawback – low sensitivity.

The acquisition of multiple numbers of charge states for peptides in the MS source can result in significant sensitivity loss, as usually only a single charge state is selected for quantitation. Modifying the ionisation characteristics of peptides in the source is difficult, and changing the level of normal mobile phase modifiers has minimal effects.

Supercharging reagents

The addition of supercharging reagents to mobile phases significantly shifts the charge states of peptides and proteins present in the ESI source. This enables the analyst to change the selected precursor for quantitative purposes, with potential sensitivity gains. Sensitivity can be increased if a higher charge state gives a better fragmentation profile. Selectivity can also be improved, where selecting a different precursor removes signal from co-eluting interferents.

Dr Richard Kay, Principal Scientist within Bioanalytical Sciences at LGC, will host a webinar on, Supercharging reagents – revving up peptide LC-MS analyses, on 20th July. Richard will discuss the following during the webinar:

  • A number of examples of the application of supercharging reagents to peptide LC-MS analyses
  • Impact on the quantitative performance
  • Case studies
  • Q&A


Register for the webinar to learn more about: Supercharging reagents – revving up peptide LC-MS analyses



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