Tag Archive: peptides

Jan 22

Little to LARGE: LC-MS/MS peptide method development for small molecule specialists

The development of LC-MS/MS methods for the quantitation of large molecules within biological matrices can help to overcome some of the challenges associated with immunoassay-based analyses. For example, the need to raise new antibodies against novel analytes, poor specificity due to cross reactivity with similar compounds, and for radioimmunoassays the additional safety considerations. LC-MS/MS method …

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Jul 10

Supercharging reagents – revving up peptide LC-MS analyses

Over the past 5 years, LC-MS has (arguably) become the technique of choice to quantify peptides in biological matrices. The benefits of using LC-MS as a quantitative technique are significant, however, there is one drawback – low sensitivity. The acquisition of multiple numbers of charge states for peptides in the MS source can result in …

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May 01

Metabolism of therapeutic peptides

Peptide and protein based drugs are significantly more complicated to work with than small molecules, and as yet do not have specific guidelines for their development with regards to in-vivo metabolites. The analysis of metabolites from small molecule drugs is defined by the FDA in the metabolites in safety testing guidelines (MIST, 2008). Currently, analytical …

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