Alzheimer’s disease diagnosis: the end of the guessing game?

There are currently around 850,000 people living with dementia in the UK, and the number of people affected is expected to reach 2 million by 2051. The costs associated with dementia, estimated now at £26 billion a year, are likely to treble.

Alzheimer’s disease is the most common type of dementia, affecting between 60 and 80 percent of those diagnosed. There is no known cure, with treatments limited to preserving cognitive function. Currently, there is no non-invasive method for diagnosing Alzheimer’s disease with GP’s relying on in depth cognitive tests, with clinical confidence in diagnosis typically at 70-80%.

Doctor Helping Elderly

If confident early diagnosis could be achieved through noninvasive techniques, treatment could be introduced earlier delaying the onset of memory impairment.

The solution

The development of plaques or tangles of certain proteins (β-amyloid and tau proteins) in the brain is a known feature in Alzheimer’s disease. It is also known that abnormal accumulation of metals underlies several neurodegenerative diseases. Iron, in particular, is associated with the formation of neurofibrillary tangles in the β-amyloid plaques. The recent advances in the use of Magnetic Resonance Imaging (MRI) for the earlier detection of neurological diseases require validation to ensure the integrity of the images obtained is adequate for diagnostic purposes.

Researchers at LGC, in collaboration with partners, have been working to establish a link between novel MRI scans and quantitative elemental mapping of soft tissues. A method of mapping the levels of iron in sections of the brain using laser ablation (LA) coupled to Inductively Coupled Plasma Mass Spectrometry (ICP-MS) has been developed, along with a novel calibration strategy and standard to support quantitative tissue imaging. Correlation of the metal content associated with β-amyloid protein and MRI images will help diagnosis of AD at an early stage, where preventative therapy will have greater impact.

LGC has developed a novel calibration strategy for LA-ICP-MS that produced quantitative images for iron in whole mouse brain sections (provided through collaboration with Kings College London and the University of Warwick) and compared them with results from micro x-ray fluorescence (μ-XRF) (provided through collaboration with Ghent University and the University of Warwick). The data showed good agreement in total iron concentrations for a selection of areas within the mouse brain sections. This finding supports the proposed method as a quantitative approach; the calibration strategy has been published in the Journal of Analytical Atomic Spectrometry¹.

Impact

The development of this method for quantitative imaging of iron in the brain has the potential to lead to techniques for earlier diagnosis of Alzheimer’s disease, enabling earlier intervention, therapies and treatment aimed at delaying the onset of symptoms.

Delaying the onset of neurodegenerative disorders, such as Alzheimer’s disease, by five years could halve the number of deaths from the condition, saving 30,000 lives a year and billions of pounds in treatment costs. Reducing severe cognitive impairment in the elderly by 1% pa would cancel all estimated increases in long-term care costs due to our ageing population.

The methodology will also provide deeper understanding of the early development of Alzheimer’s disease leading the way for new treatments aimed at preventing the disease.

Heidi Goenaga-Infante, Principal Scientist for inorganic analysis at LGC, commented: “This cutting-edge research is already proving to be of significant benefit to the validation of non-invasive diagnostic tools for Alzheimer’s disease. The potential for metal imaging mass spectrometry of other biological tissues to probe the reported links between metals and disease states is now a step closer.”

If you’d like to learn more about our work and read other case studies, visit our website.

¹ J O’Reilly, D Douglas, J Braybrook, P.-W. So, E Vergucht, J Garrevoet, B Vekemans, L Vinczec and H Goenaga-Infante, “A novel calibration strategy for the quantitative imaging of iron in biological tissues by LA-ICP-MS using matrix-matched standards and internal standardisation”, J Anal. At. Spectrom., 2014, 29, 1378-1384

World Immunization Week 2018: Why and how #VaccinesWork

This week is World Immunization Week, a global campaign to raise awareness of infectious diseases and to educate the public on the importance of vaccination.

Vaccines are a relatively modern tool, with the world’s first successful vaccine being developed in 1796 for smallpox.  Numbers show that when vaccinations steadily increase, rates of death from diseases like measles and polio were vastly reduced.

via WHOMeasles is a highly contagious disease and remains one of the leading causes of death for young children around the world. Before the first vaccine for measles was introduced in 1963, the disease caused 2.6 million deaths each year. However, between 2000 and 2016, the global death rate from measles was decreased by 84%, falling below 100,000 deaths annually for the first time.

Similarly, cases of polio have fallen 99% since the launch of the Global Polio Eradication Initiative in 1988, nearly achieving its goal of eradicating the disease entirely.

This illustrates that vaccinations aren’t just important to the people who take them: over time the use of vaccinations can protect entire populations from contagious disease with what’s known as herd immunity, or ‘community immunity’. Vaccines build immunity in individuals by mimicking an infection. The body’s immune system kicks in and learns to fight that particular infection, achieving immunity to that strain of disease.

In larger populations, the number of new infections decreases as individuals are vaccinated and go through this process. It’s more difficult for diseases to spread if more members of the population can’t be infected. This disrupts the wildfire-like spread of contagions and even protects more vulnerable members of the population who aren’t immune yet, like children, or cannot become immune due to medical reasons.  This only works if enough people get vaccinated though.

Making the world safer against viruses and bacteria is an important step for the future of our communities, which is why our scientists work so hard to help support immunization around the globe. We hope to play a part in the eradication of deadly illnesses by using our research capabilities to progress current research. 

Using mass spectrometry, genotyping technology, DNA/RNA extraction technology, and Next Generation Sequencing, our teams generate a broader understanding of the genetics of diseases, as well as how particular molecules behave and are characterised.

We develop biomaterials that enable researchers to develop vaccines for epidemic diseases such as the Zika and Ebola viruses. Our reference materials are used to prove the quality and purity of medicines, while our microbiology teams have a strong reputation in anti-infective research, as well as antimicrobial surveillance and drug development.

There’s still a long way to go, but in the meantime, visit the World Health Organisation’s website to learn about how vaccines work and how you can help.

Finding harmony in newborn blood spot screening

Every forty seconds, a baby is born in the UK. That’s nearly 775,000 births across the United Kingdom in 2016 alone. It’s important that each of these children is given their best chance at a healthy future from the moment they are born.

Currently, all parents of newborns in the UK are offered newborn blood spot screening, a test which detects nine conditions and inherited diseases, including cystic fibrosis, congenital hypothyroidism, and sickle cell disease. The level of hormones or amino acids in the blood at the time the sample is taken leads to early detection. The goal is to detect and treat conditions before they cause severe developmental problems or unnecessary suffering so children can lead as normal lives as possible.

With the number of infants tested each year and the use of nationally agreed protocols with specified cut-off values, harmonisation of methods across the 14 laboratories performing these tests is extremely vital.  Each time a sample is analysed, it should produce the same results. The cost and time of retesting samples can be great and can cause unnecessary stress to the families at an already challenging time.  Additionally, the network of newborn screening laboratories in the UK should have access to the newest, most accurate methods and data.

This is why we have partnered with Dr Rachel Carling, one of the country’s foremost authorities on newborn screening, and the NHS England as part of the CSO’s Knowledge Transfer Partnership (KTP), a programme that teams up leaders in healthcare with the UK National Measurement System’s lab, including the National Measurement Laboratory (NML) at LGC, to solve measurement challenges in their fields.

Through the partnership, we plan to help create methods and materials that will lead to greater harmonisation and provide a framework within which more analytes can be added to the UK’s screening programme to be able to test for new diseases at birth.

As part of the KTP, LGC’s Chris Hopley and Simon Cowen will be discussing best practice in newborn screening with the network of labs at a workshop in London this week. Together, we hope to help deliver greater efficiency and certainty for these children and their families.